| Name | Character | Episodes |
|---|---|---|
| Samira Wiley | Self - Presenter | 2 |
| Joe Biden | Self | 2 |
| Kelly Osbourne | Self - Presenter | 2 |
| Billy Porter | Self - Speaker | 2 |
| Lily Tomlin | Self - Speaker | 1 |
| Daniel Radcliffe | Self - Presenter | 1 |
| Emma Stone | Self - Presenter | 1 |
| Julia Roberts | Self | 1 |
| Ian McKellen | Self - Speaker | 1 |
| Angela Bassett | Self | 1 |
| Debra Messing | Self | 1 |
| Tyler Posey | Self - Presenter | 1 |
| Miley Cyrus | Self - Performer | 1 |
| Alyssa Milano | Self - Presenter | 1 |
| Adam Lambert | Self - Performer | 1 |
| Jack Antonoff | Self - Performer | 1 |
| Frankie Grande | Self - Presenter | 1 |
| Cheyenne Jackson | Self - Speaker | 1 |
| Michaela Jaé Rodriguez | Self - Presenter | 1 |
| Lance Bass | Self - Performer | 1 |
| Danielle Brooks | Self - Honoree | 1 |
| Ryan Murphy | Self - Honoree | 1 |
| Sara Ramirez | Self - Presenter | 1 |
| Mindy Kaling | Self - Presenter | 1 |
| Rita Ora | Self - Performer | 1 |
| Barack Obama | Self (archive footage) | 1 |
| Sia | Self - Performer | 1 |
| Joe Manganiello | Self - Presenter | 1 |
| Judith Light | Self - Speaker | 1 |
| David Thornton | Self | 1 |
| Cyndi Lauper | Self - Honoree | 1 |
| Ava DuVernay | Self - Honoree | 1 |
| Joel Grey | Self - Presenter | 1 |
| Samira Wiley | Self - Honoree | 1 |
| Jesse Tyler Ferguson | Self | 1 |
| Harvey Fierstein | Self - Honoree | 1 |
| RuPaul | Self - Speaker | 1 |
| Darren Criss | Self | 1 |
| Jen Richards | Self - Red Carpet Host | 1 |
| Logan Browning | Self - Speaker | 1 |
| Will McCormack | Self | 1 |
| Bebe Rexha | Self - Performer | 1 |
| Adam Levine | Self - Performer | 1 |
| Elle King | Self - Performer | 1 |
| Cher | Self - Presenter | 1 |
| Raven-Symoné | Self - Presenter | 1 |
| Jane Fonda | Self - Speaker | 1 |
| Taryn Manning | Self - Honoree | 1 |
| Laverne Cox | Self - Honoree | 1 |
| Demi Lovato | Self - Performer | 1 |
| Elizabeth Warren | Self | 1 |
| Barry Jenkins | Self - Speaker | 1 |
| Lea DeLaria | Self - Honoree | 1 |
| Lea DeLaria | Self - Presenter | 1 |
| Padma Lakshmi | Self - Presenter | 1 |
| Michael Stipe | Self | 1 |
| Eureka O'Hara | Self | 1 |
| Nancy Pelosi | Self - Honoree | 1 |
| Nancy Pelosi | Self - Speaker | 1 |
| Julia Michaels | Self | 1 |
| Carson Kressley | Self | 1 |
| Alex Newell | Self - Performer | 1 |
| Alyssa Edwards | Self - Performer | 1 |
| Dominique Jackson | Self - Presenter | 1 |
| Alaska Thunderfuck | Self - Performer | 1 |
| Margaret Atwood | Self - Honoree (archive footage) | 1 |
| Billie Jean King | Self - Honoree | 1 |
| Nate Ruess | Self | 1 |
| Anita Hill | Self - Presenter | 1 |
| Diane Warren | Self | 1 |
| Tituss Burgess | Self - Presenter | 1 |
| Tituss Burgess | Self | 1 |
| Joshua Allan Eads | Self - Performer | 1 |
| Bob the Drag Queen | Self - Presenter | 1 |
| Hayley Kiyoko | Self - Performer | 1 |
| Laura Jane Grace | Self - Presenter | 1 |