| Name | Character | Episodes |
|---|---|---|
| Spencer Jones | Kempe | 18 |
| Dominic Coleman | Condell | 18 |
| Mark Heap | Sir Robert Greene | 18 |
| Liza Tarbuck | Anne Hathaway | 18 |
| Gemma Whelan | Kate | 18 |
| Paula Wilcox | Mary Arden | 18 |
| David Mitchell | Will Shakespeare | 18 |
| Harry Enfield | John Shakespeare | 18 |
| Steve Speirs | Burbage | 18 |
| Jocelyn Jee Esien | Lucy | 7 |
| Emma Thompson | Queen Elizabeth I | 1 |
| Kenneth Branagh | Colin/The Stranger | 1 |
| Beattie Edmondson | Toby | 1 |
| Ben Miller | Wolf Hall | 1 |
| Noel Fielding | Thomas Morley | 1 |
| Lily Cole | Ephie Watkins | 1 |
| Margaret Clunie | Silvia | 1 |
| Adrian Edmondson | Dogberry | 1 |
| Geoffrey Whitehead | Hunt | 1 |
| Steve Toussaint | Otello | 1 |
| Gabrielle Glaister | Judge | 1 |
| Geoffrey McGivern | Sir Thomas | 1 |
| Tom Edden | Servant | 1 |
| Tom Edden | Clerk | 1 |
| Mina Anwar | Weird sister | 1 |
| Karl Theobald | Job Scratchit | 1 |
| Sarah Finigan | Weird sister | 1 |
| Lizzie Winkler | Gertrude | 1 |