| Name | Character | Episodes |
|---|---|---|
| Matthew Lillard | Shaggy Rogers (voice) | 53 |
| Frank Welker | Fred Jones / Scooby-Doo (voice) | 53 |
| Grey DeLisle | Daphne Blake (voice) | 53 |
| Kate Micucci | Velma Dinkley (voice) | 53 |
| Mark Hamill | General Stall (voice) | 1 |
| Laraine Newman | Professor Salazar / Running Girl / Female Security Guard (voice) | 1 |
| Dee Bradley Baker | Professor / Sobek / Duncan (voice) | 1 |
| Liliana Mumy | Daughter (voice) | 1 |
| JB Blanc | Vic / Colander (voice) | 1 |
| Phil Morris | Dunsbury (voice) | 1 |
| Chord Overstreet | Mitchell / Andrew (voice) | 1 |
| Candi Milo | Ms. Tuckle (voice) | 1 |
| David Kaye | Bradwick / Headless Count (voice) | 1 |
| Julie Nathanson | Kimmy / Bride’s Mother (voice) | 1 |
| Ben Diskin | Additional Voices (voice) | 1 |
| Christian Lanz | Miguel (voice) | 1 |
| Edward Bosco | Werewolf (voice) | 1 |