| Name | Character | Episodes |
|---|---|---|
| Lily Du | Self - Host | 43 |
| Ify Nwadiwe | Self - Guest | 3 |
| Erika Ishii | Self - Guest | 2 |
| Anna Garcia | Self - Guest | 2 |
| Katie Marovitch | Self - Guest | 2 |
| Rekha Shankar | Self - Guest | 2 |
| Sam Reich | Self - Guest | 2 |
| Ally Beardsley | Self - Guest | 2 |
| Raphael Chestang | Self - Guest | 2 |
| Oscar Montoya | Self - Guest | 2 |
| Mike Trapp | Self - Guest | 2 |
| Jessica Ross | Self - Guest | 2 |
| David Cyr Kerns | Self - Guest | 1 |
| Milana Vayntrub | Self - Guest | 1 |
| Bex Taylor-Klaus | Self - Guest | 1 |
| Bex Taylor-Klaus | Self (archive footage) | 1 |
| Josh Ruben | Self - Guest | 1 |
| Betsy Sodaro | Self - Guest | 1 |
| Angela Giarratana | Self - Guest | 1 |
| Gillian Vigman | Self - Guest | 1 |
| Andrew Lawrence | Self - Guest | 1 |
| Zac Oyama | Self - Guest | 1 |
| Vic Michaelis | Self - Guest | 1 |
| Dhruv Uday Singh | Self - Guest | 1 |
| Michelle Khare | Self - Guest | 1 |
| Eugene Cordero | Self - Guest | 1 |
| Paul F. Tompkins | Self - Guest | 1 |
| Kimia Behpoornia | Self - Guest | 1 |
| Ricky Ullman | Self - Guest | 1 |
| Desmond Chiam | Self - Guest | 1 |
| Anjali Bhimani | Self - Guest | 1 |
| Tawny Newsome | Self - Guest | 1 |
| Erika Ishii | Self (archive footage) | 1 |
| Lilan Bowden | Self - Guest | 1 |
| Matthew Mercer | Self - Guest | 1 |
| Shayne Topp | Self - Guest | 1 |
| Brennan Lee Mulligan | Self - Guest | 1 |
| Blu del Barrio | Self - Guest | 1 |
| Blu del Barrio | Self (archive footage) | 1 |
| Sandeep Parikh | Self - Guest | 1 |
| Katya Zamolodchikova | Self - Guest | 1 |
| Katie Marovitch | Self (archive footage) | 1 |
| Shakira Ja'nai Paye | Self - Guest | 1 |
| Elaine Carroll | Self - Guest | 1 |
| Siobhan Thompson | Self - Guest | 1 |
| Davida Williams | Self - Guest | 1 |
| Ify Nwadiwe | Self (archive footage) | 1 |
| Zeke Nicholson | Self - Guest | 1 |
| Monét X Change | Self - Guest | 1 |
| Sam Reich | Self (archive footage) | 1 |
| Alaska Thunderfuck | Self - Guest | 1 |
| Sam Riegel | Self - Guest | 1 |
| Chanse McCrary | Self - Guest | 1 |
| Ally Beardsley | Self (archive footage) | 1 |
| Raphael Chestang | Self (archive footage) | 1 |
| Christine Medrano | Self - Guest | 1 |
| Amanda Lehan-Canto | Self - Guest | 1 |
| Jon Gabrus | Self - Guest | 1 |
| Chris Grace | Self - Guest | 1 |
| Kassem Gharaibeh | Self - Guest | 1 |
| Jiavani | Self - Guest | 1 |
| Kalilah Harris | Self - Guest | 1 |
| Jacob Wysocki | Self - Guest | 1 |
| Jacob Wysocki | Self (archive footage) | 1 |
| Sam Denby | Self - Guest | 1 |
| Mica Burton | Self - Guest | 1 |
| Jujubee | Self - Guest | 1 |
| Arasha Lalani | Self - Guest | 1 |
| Oscar Montoya | Self (archive footage) | 1 |
| Carl Tart | Self - Guest | 1 |
| Noah Grossman | Self - Guest | 1 |
| Lisa Gilroy | Self - Guest | 1 |
| Jessica Ross | Self (archive footage) | 1 |
| Ele Woods | Self - Guest | 1 |
| Ele Woods | Self (archive footage) | 1 |
| Alyssa Limperis | Self - Guest | 1 |
| Demi Adejuyigbe | Self - Guest | 1 |
| Fiona Nova | Self - Guest | 1 |
| Ross Bryant | Self - Guest | 1 |
| Adam Conover | Self - Guest | 1 |
| Parvati Shallow | Self - Guest | 1 |
| Noël Wells | Self - Guest | 1 |
| Brian David Gilbert | Self - Guest | 1 |
| Brian David Gilbert | Self (archive footage) | 1 |
| Whitney Moore | Self - Guest | 1 |
| Adam Chase | Self - Guest | 1 |
| Jacquis Neal | Self - Guest | 1 |